Were obtained from milk, cheese as well as other dairy goods from a single traditional sheepfarm in Slovakia. The Table 1 shows selected (67) meals samples includingTable 1 Number of analyzed samples and Salmonella constructive samplesType of samples Milk Cheese Other dairy merchandise Total Enterobacteriaceae Analyzed samples 21 25 21 67 Salmonella spp. Positive samples 4/21 0/25 0/21 4/JMBFS / Hleba et al. 2011 1 (1) 1-milk (n = 21), cheese (n = 25) as well as other dairy merchandise (whey, boiled whey, sheep cheese) (n = 21). The samples were collected by sterile cotton swabs (Copan Inovation, Brescia) and transported for the laboratory (SUA in Nitra, Division of Microbiology).Enterobacteriaceae genera and Salmonella spp. isolations had been performed by a conventional plating process. The initial step was completed around the MacConkey agar (Biomark, Pune) for Enterobacteriaceae genera. Incubation was performing for 24 hours at 37 . After incubation on the MacConkey agar, we used Chromogenic coliform agar (Biolife, Italiana), XLD agar (Biolife, Italiana) and SS agar (MkB test, Rosina) and we chose the streak plate (four-ways) strategy for getting the pure colonies. Incubation was carried out for 24 hours at 37 . This step was repeating till we had absolutely cleaned culture of Salmonella spp. as well as other strains from Enterobacteriaceae genera. Right after the incubation and identification it was isolated 13 colonies of Salmonella spp. of four positive samples from milk.The biochemical identification of Salmonella spp.Approach on the Triple sugar iron agar (Biolife, Italiana) for the fundamental biochemical identification of Salmonella spp. and MIP-1 alpha/CCL3 Protein Mouse ENTEROtest 24 (Pliva-Lachema, Brno), like TNW Lite 7.0 identification application (Pliva-Lachema, Brno) for far more detailed biochemical identification was used. Preparation of indentification plates of ENTEROtest 24 was completed inside the Laminaire box (Ads Laminaire, Le Pre-Saint Gervais) to make sure the higher sterility, significantly less risk of contaminations from air and for precise final results. Functioning process of ENTEROtest 24 is described inside the competent manual.The isolation of DNA from Salmonella spp.The pure colonies of Salmonella spp. had been subjected to DNA isolation applying PrepSEQTM Fast Spin Sample Preparation Kit (Applied Biosystem, USA). Total functioning procedure is described in the kit manual.Basic Sample Preparation ProtocolSample of 750 L was loaded onto the spin column and microcentrifuged for 3 minutes at maximum speed (12000 rpm). Supernatant was discarded and 50 L of Lysis Buffer was added for the pellet. Samples were incubated for ten minutes at 95 . The samples afterJMBFS / Hleba et al. 2011 1 (1) 1-incubation were added to cool for two min at area temperature. Then have been added 250 l of water to samples. After the samples have been centrifuged one minute at maximum speed (12000 rpm).Identification of Salmonella spp. by True time PCR Step ONEReal time PCR (Applied Biosystem, USA) for a genetic confirmation of belonging to the genus Salmonella spp MicroSEQSalmonella spp. Detection Kit (Applied Biosystem, USA) was used for the actual PCR reaction. Total data is described inside the kit manual.Antimicrobial susceptibility testingAntimicrobial susceptibility testing was done by disk diffusion method (according EUCAST (2009) European committee on antimicrobial susceptibility testing). Antibiotic disks were applied (Oxoid, England). The pure Carbonic Anhydrase 1 Protein Human inoculum of strain of Salmonella spp. and strains from Enterobacteriaceae genera was ready by suspending of colonies fro.