Z-stacks (150 cells for each condition and time point) comprising the total height of the cells were acquired (X sections at 0.5 mm intervals) to measure EGFP and anti-VSVG fluorescence as described for the Golgi analysis

Before immunostaining, blots ended up stained for overall protein with Ponceau S (Sigma) they had been then incubated with antibodies diluted in TBS+5% milk+.1% Tween. Peroxidase-conjugated secondary antibodies ended up…

Focusing on injuries and health effects potentially allows us to capture the medically relevant aspect of chemical injuries, without resorting to large-scale in vivo characterizations of the multitude of potentially harmful chemicals we encounter in the environment

Concentrating on accidents and overall health outcomes perhaps permits us to capture the medically pertinent aspect of chemical accidents, with out resorting to huge-scale in vivo characterizations of the multitude…