Ting Details Author Contributions Conceived and made the experiments: DK JDZ MLF JLF. Performed the experiments: DK AEKH MLF JLF. Analyzed the information: DK JLF JDZ. Contributed reagents/materials/analysis tools: DK AEKH YD JW MLF. Wrote the paper: DK JLF JDZ. References 1. Freegard TJ The physical basis of transparency of your typical cornea. Eye 11: 465471. 2. Garana RM, Petroll WM, Chen WT, Herman IM, Barry P, et al. Radial keratotomy. II. Part in the myofibroblast in NT 157 web corneal wound contraction. Invest Ophthalmol Vis Sci 33: 32713282. 3. Moller-Pedersen T, Li HF, Petroll WM, Cavanagh HD, Jester JV Confocal microscopic characterization of wound repair just after photorefractive keratectomy. Invest Ophthalmol Vis Sci 39: 487501. four. Beales MP, Dimethylenastron cost Funderburgh JL, Jester JV, Hassell JR Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: maintenance of your keratocyte phenotype in culture. 22948146 Invest Ophthalmol Vis Sci 40: 16581663. five. Funderburgh JL, Funderburgh ML, Mann MM, Corpuz L, Roth MR Proteoglycan expression during transforming growth issue beta -induced keratocyte-myofibroblast transdifferentiation. J Biol Chem 276: 4417344178. six. Funderburgh JL, Funderburgh ML, Mann MM, Prakash S, Conrad GW Synthesis of corneal keratan sulfate proteoglycans by bovine keratocytes in vitro. J Biol Chem 271: 3143131436. 7. Funderburgh JL, Mann MM, Funderburgh ML Keratocyte phenotype mediates proteoglycan structure: a part for fibroblasts in corneal fibrosis. J Biol Chem 278: 4562945637. 8. Extended CJ, Roth MR, Tasheva ES, Funderburgh M, Smit R, et al. Fibroblast growth 1317923 factor-2 promotes keratan sulfate proteoglycan expression by keratocytes in vitro. J Biol Chem 275: 1391813923. 9. Berryhill BL, Kader R, Kane B, Birk DE, Feng J, et al. Partial restoration on the keratocyte phenotype to bovine keratocytes created fibroblastic by serum. Invest Ophthalmol Vis Sci 43: 34163421. ten. Hassell JR, Birk DE The molecular basis of corneal transparency. Exp Eye Res 91: 326335. 11. Musselmann K, Alexandrou B, Kane B, Hassell JR Upkeep on the keratocyte phenotype for the duration of cell proliferation stimulated by insulin. J Biol Chem 280: 3263432639. 12. Guo X, Hutcheon AE, Melotti SA, Zieske JD, Trinkaus-Randall V, et al. Morphologic characterization of organized extracellular matrix deposition by ascorbic acid-stimulated human corneal fibroblasts. Invest Ophthalmol Vis Sci 48: 40504060. 10 Substratum-Induced Organization of Corneal ECM 13. Karamichos D, Guo XQ, Hutcheon AE, Zieske JD Human corneal fibrosis: an in vitro model. Invest Ophthalmol Vis Sci 51: 13821388. 14. Ren R, Hutcheon AE, Guo XQ, Saeidi N, Melotti SA, et al. Human key corneal fibroblasts synthesize and deposit proteoglycans in long-term 3D cultures. Dev Dyn 237: 27052715. 15. Karamichos D, Hutcheon AE, Zieske JD Transforming development factorbeta3 regulates assembly of a non-fibrotic matrix within a 3D corneal model. J Tissue Eng Regen Med 5: e228238. 16. Du Y, Funderburgh ML, Mann MM, SundarRaj N, Funderburgh JL Multipotent stem cells in human corneal stroma. Stem Cells 23: 12661275. 17. Du Y, Sundarraj N, Funderburgh ML, Harvey SA, Birk DE, et al. Secretion and organization of a cornea-like tissue in vitro by stem cells from human corneal stroma. Invest Ophthalmol Vis Sci 48: 50385045. 18. Wu J, Du Y, Watkins SC, Funderburgh JL, Wagner WR The engineering of organized human corneal tissue through the spatial guidance of corneal stromal stem cells. Biomaterials 33: 13431352. 19. Wu J, Du Y, Mann MM, Yan.Ting Details Author Contributions Conceived and designed the experiments: DK JDZ MLF JLF. Performed the experiments: DK AEKH MLF JLF. Analyzed the data: DK JLF JDZ. Contributed reagents/materials/analysis tools: DK AEKH YD JW MLF. Wrote the paper: DK JLF JDZ. References 1. Freegard TJ The physical basis of transparency in the normal cornea. Eye 11: 465471. 2. Garana RM, Petroll WM, Chen WT, Herman IM, Barry P, et al. Radial keratotomy. II. Function on the myofibroblast in corneal wound contraction. Invest Ophthalmol Vis Sci 33: 32713282. three. Moller-Pedersen T, Li HF, Petroll WM, Cavanagh HD, Jester JV Confocal microscopic characterization of wound repair following photorefractive keratectomy. Invest Ophthalmol Vis Sci 39: 487501. four. Beales MP, Funderburgh JL, Jester JV, Hassell JR Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: upkeep from the keratocyte phenotype in culture. 22948146 Invest Ophthalmol Vis Sci 40: 16581663. five. Funderburgh JL, Funderburgh ML, Mann MM, Corpuz L, Roth MR Proteoglycan expression for the duration of transforming growth issue beta -induced keratocyte-myofibroblast transdifferentiation. J Biol Chem 276: 4417344178. 6. Funderburgh JL, Funderburgh ML, Mann MM, Prakash S, Conrad GW Synthesis of corneal keratan sulfate proteoglycans by bovine keratocytes in vitro. J Biol Chem 271: 3143131436. 7. Funderburgh JL, Mann MM, Funderburgh ML Keratocyte phenotype mediates proteoglycan structure: a part for fibroblasts in corneal fibrosis. J Biol Chem 278: 4562945637. eight. Lengthy CJ, Roth MR, Tasheva ES, Funderburgh M, Smit R, et al. Fibroblast development 1317923 factor-2 promotes keratan sulfate proteoglycan expression by keratocytes in vitro. J Biol Chem 275: 1391813923. 9. Berryhill BL, Kader R, Kane B, Birk DE, Feng J, et al. Partial restoration from the keratocyte phenotype to bovine keratocytes made fibroblastic by serum. Invest Ophthalmol Vis Sci 43: 34163421. 10. Hassell JR, Birk DE The molecular basis of corneal transparency. Exp Eye Res 91: 326335. 11. Musselmann K, Alexandrou B, Kane B, Hassell JR Maintenance with the keratocyte phenotype throughout cell proliferation stimulated by insulin. J Biol Chem 280: 3263432639. 12. Guo X, Hutcheon AE, Melotti SA, Zieske JD, Trinkaus-Randall V, et al. Morphologic characterization of organized extracellular matrix deposition by ascorbic acid-stimulated human corneal fibroblasts. Invest Ophthalmol Vis Sci 48: 40504060. ten Substratum-Induced Organization of Corneal ECM 13. Karamichos D, Guo XQ, Hutcheon AE, Zieske JD Human corneal fibrosis: an in vitro model. Invest Ophthalmol Vis Sci 51: 13821388. 14. Ren R, Hutcheon AE, Guo XQ, Saeidi N, Melotti SA, et al. Human major corneal fibroblasts synthesize and deposit proteoglycans in long-term 3D cultures. Dev Dyn 237: 27052715. 15. Karamichos D, Hutcheon AE, Zieske JD Transforming growth factorbeta3 regulates assembly of a non-fibrotic matrix within a 3D corneal model. J Tissue Eng Regen Med 5: e228238. 16. Du Y, Funderburgh ML, Mann MM, SundarRaj N, Funderburgh JL Multipotent stem cells in human corneal stroma. Stem Cells 23: 12661275. 17. Du Y, Sundarraj N, Funderburgh ML, Harvey SA, Birk DE, et al. Secretion and organization of a cornea-like tissue in vitro by stem cells from human corneal stroma. Invest Ophthalmol Vis Sci 48: 50385045. 18. Wu J, Du Y, Watkins SC, Funderburgh JL, Wagner WR The engineering of organized human corneal tissue via the spatial guidance of corneal stromal stem cells. Biomaterials 33: 13431352. 19. Wu J, Du Y, Mann MM, Yan.