Ng exposure to simazine for (p 0.05). simazine for 48 h compared together with the controls48 h compared using the controls (p 0.05).two.three. Effects of Simazine on Protein Expression in MN9D Cells 2.three. Effects of Simazine on Protein Expression in MN9D Cells The levels of DYT5b, AADC, DAT, VMAT2, MAO and COMT protein expression in the levels of DYT5b, AADC, DAT, VMAT2, MAO and COMT protein expression in simazinesimazine-treated MN9D cells were determined. We analyzed the primary effects of exposure dose, treated MN9D cells have been determined. We analyzed the key effects of exposure dose, exposure time exposure time as well as the interaction of these two variables (Figures 3). plus the interaction of these two elements (Figures three). Figure three shows the impact of exposure to simazine for 12 and 24 h on the protein expression levels Figure 3 shows the impact of exposure to simazine for 12 and 24 h on the protein expression levels of DYT5b (a as well as a ) and AADC (b and b ).PTH Protein Biological Activity DYT5b protein levels in cells exposed to 300 and 600 of DYT5b (a and a’) and AADC (b and b’). DYT5b protein levels in cells exposed to 300 and 600 simazine for 12 h have been considerably enhanced compared with all the handle (p 0.05) (a in addition to a ); AADC simazine for 12 h have been considerably elevated compared together with the handle (p 0.05) (a and a’); AADC protein levels in cells exposure to 600 simazine for 12 h was significantly elevated compared with protein levels in cells exposure to 600 simazine for 12 h was significantly enhanced compared the handle (p 0.05) (b and b ); DYT5b and AADC protein levels in cells exposed to 300 and 600 simazine for 24 h have been considerably elevated compared using the controls (p 0.05). DYT5b, AADCInt. J. Mol. Sci. 2017, 18, 2404 Int. Int. J. Mol. Sci. 2017, 18, 2404 J. Mol. Sci. 2017, 18,4 of 13 four of 13 13 four ofwith the control (p 0.05) (b and b’); DYT5b and AADC protein levels in cells exposed to 300 and 600 with all the handle (p h were drastically elevated compared levels in controls (p 0.05). and 600 simazine for 24 0.05) (b and b’); DYT5b and AADC protein using the cells exposed to 300 DYT5b, proteinsimazine for 24 hexposure exposure to600 simazinesimazinewere all significantly decreased levels following were considerably improved 600 for 48 the controls (p all drastically AADC protein levels following to 300 and 300 and compared withh for 48 h had been 0.Chk1 Protein Molecular Weight 05).PMID:36628218 DYT5b, AADC using the controls (p controls comparedprotein levelswith the 0.05). (p to 300 and 600 simazine for 48 h have been all drastically decreased compared following exposure 0.05). decreased compared with the controls (p 0.05).Figure three. The effects of exposure to simazine for 12, 24, 48 h around the expression of DYT5b (a,a’), AADC Figure three. The effects of exposure to simazine for 12, 24, 48 h on the expression of DYT5b (a,a ), AADC Figure three. The effects of cells determined with a western blotting analysis. Bars indicate imply S.E.M. (b,b’) protein in MN9D exposure to simazine for 12, 24, 48 h on the expression of DYT5b (a,a’), AADC (b,b ) protein in in MN9D cells determined withaawestern blotting evaluation. Bars indicate mean S.E.M. MN9D cells determined with western blotting analysis. Bars indicate mean S.E.M. (b,b’) proteinsignificant difference compared with all the control, p 0.05, 3 repeated experiments for statistically statistically considerable difference compared using the control, p 0.05, 33repeated experiments for p 0.05, repeated experiments for statistically = 3. each and every group, ns.