Tions NAO developed the experimental tactic and plasmid design, performed cloning
Tions NAO developed the experimental strategy and plasmid style, performed cloning procedures, and drafted the manuscript. SVK performed the cell culture experiments and helped to draft the manuscript. IIV initiated the project, participated in its design and style and coordination and drafted the manuscript. NAO, JAH and IIV carried out the copy number determination experiments, SVK, JAH and IIV performed eGFP level determinations. AGG and KGS coordinated the project. All of the authors have read and authorized the final manuscript. Acknowledgments The authors want to thank Dr Sergei Khaidukhov for performing the FACS analysis and Dr Ivan Vorobiev (senior) for supplying the suggestions for FACS data interpretation. We also thank Dr Ivan Zvyagin, Lev Usakin, Maria Kordyukova and Victoria Shender for taking component within the molecular cloning procedures. The function was supported by the following grants: RFBR 13-0440277- “Combinatorial chemistry and biology approaches to theranostics of various sclerosis”; RFBR 14-04-00647 ” Combinatorial biology approaches applying yeast show for improvement of biocatalysts de novo”; System on the Presidium with the Russian Academy of Sciences No24 “Detection of Viral Antigens as you can Triggers of Autoimmune Neurodegeneration and MMP Gene ID Nanotechnologies and Nanomaterials”; Program with the Presidium of Russian Academy of Sciences “Fundamental science for medicine” – grant “Features ofOrlova et al. BMC Biotechnology 2014, 14:56 biomedcentral.com/1472-6750/14/Page 11 of18. Wajih N, Hutson SM, Owen J, Wallin R: Improved production of functional recombinant human clotting factor IX by child hamster kidney cells engineered to overexpress VKORC1, the vitamin K two,3-epoxide-reducing enzyme with the vitamin K cycle. J Biol Chem 2005, 280(36):316031607. 19. Bebbington CR, Hentschel CC: The use of vectors determined by gene amplification for the expression of cloned genes in mammalian cells. In DNA Cloning. Volume IIIth edition. Edited by Glover D. San Diego: Academic; 1987:16388.doi:10.1186/1472-6750-14-56 Cite this article as: Orlova et al.: Enhanced elongation factor-1 alpha-based vectors for steady high-level expression of heterologous proteins in Chinese hamster ovary cells. BMC Biotechnology 2014 14:56.Submit your next manuscript to BioMed Central and take complete benefit of:Convenient on-line submission Thorough peer critique No space constraints or colour figure charges Quick publication on acceptance Inclusion in PubMed, CAS, Scopus and Google Scholar Analysis which is freely obtainable for redistributionSubmit your manuscript at biomedcentral.com/submit
Redox Biology two (2014) 273Contents lists available at ScienceDirectRedox Biologyjournal homepage: elsevier.com/AT1 Receptor Antagonist list locate/redoxResearch PaperMitochondria-targeted heme oxygenase-1 induces oxidative anxiety and mitochondrial dysfunction in macrophages, kidney fibroblasts and in chronic alcohol hepatotoxicitySeema Bansal, Gopa Biswas 1, Narayan G. Avadhani nThe Department of Animal Biology as well as the Mari Lowe Center for Comparative Oncology, College of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USAart ic l e i nf oArticle history: Received 2 July 2013 Received in revised kind 16 July 2013 Accepted 16 July 2013 Out there on the net 23 July 2013 Keywords: Heme oxygenase-1 Mitochondrial targeting Cytochrome c Oxidase Heme aa3 content ROS production Autophagya b s t r a c tThe inducible type of Heme Oxygenase-1 (HO-1), a major endoplasmic reticulum (ER) related heme protein, is.