ion have been were significantly changed (log2 2). Among them, the expression levels of GA synthesis associated genes had been drastically reduced, such as GA20ox (Traes1A01G263600 and Traes3D01G401400), which encoded the crucial enzymes in the GA synthetic pathway, and terpene synthetase gene (TraesCS7A01G002400) was decreased about 2.1, four.2, and 14.5 instances asInt. J. Mol. Sci. 2021, 22, x FOR PEER REVIEW6 ofInt. J. Mol. Sci. 2021, 22,substantially changed (log2 2). Amongst them, the expression levels of GA synthesis 6 of 12 related genes had been substantially lowered, for example GA20ox (Traes1A01G263600 and Traes3D01G401400), which encoded the essential enzymes in the GA synthetic pathway, and terpene synthetase gene (TraesCS7A01G002400) was decreased about two.1, 4.two, and 14.5 occasions as compared with WT (Figure 4A), respectively. However, the of GA2ox compared with WT (Figure 4A), respectively. Alternatively, the expressionexpression of GA2ox (TraesCS3B01G166100) and gene (TraesCS6D01G166900, TraesCS6B01G074100 (TraesCS3B01G166100) and cytochrome P450cytochrome P450 gene (TraesCS6D01G166900, and CYP1 Activator Species TraesCS5D01G323800) TraesCS5D01G323800) which associated to GA inactivation,DELLA TraesCS6B01G074100 and which connected to GA inactivation, as well as the expression of along with the (TraesCS1A01G413800 and TraesCS3B01G262400), the vital adverse the important expression of DELLA (TraesCS1A01G413800 and TraesCS3B01G262400), regulators of GA signal HSP90 Antagonist Formulation transduction pathway,transduction pathway, had been substantially enhanced with adverse regulators of GA signal have been considerably elevated with 1.four, 1.six, 1.five, 1.three, 1.4 and 1.6, 1.5, 1.three, 1.four and 1.four occasions of WT,than that of WT, respectively. In addition, it was 1.4, 1.4 occasions larger than that greater respectively. Moreover, it was identified that the expression levelsexpression levels of GA signal transduction genes, and identified that the of GA signal transduction genes, TPR (TraesCS6B01G005400 TPR TraesCS4A01G244800) wereTraesCS4A01G244800)occasions decreased and 2.two occasions (p(p 0.01) (TraesCS6B01G005400 and decreased about 3.3 had been (p 0.01) about 3.three occasions 0.01) compared with(p 0.01) outcomes indicated thatThe final results indicated that TaWUS-like and and two.two occasions WT. The compared with WT. TaWUS-like could inhibit the synthesis could functiontheGA, which and function of GA, which was constant with the phenotype of inhibit of synthesis was consistent together with the phenotype of dwarfing plants with a decrease GA content in internodes. dwarfing plants having a decrease GA content material in internodes.Figure four. Expression levels the downstream GA-, BR- (A) and cell division-related genes (B) regulated by by TaWUS-likeFigure 4. Expression levels ofof the downstream GA-, BR- (A) and cell division-related genes (B) regulatedTaWUS-like-OE. OE. Red and green circles indicate the expression with the genes was changed upward and downward, respectively. Red and green circles indicate the expression with the genes was changed upward and downward, respectively.In addition, the expression levels of genes involved inside the BR pathway in which five In addition, the expression levels of genes involved in the BR pathway in which five genesrelated to synthesis and 3 connected to signal transduction were also changed siggenes related to synthesis and three connected to signal transduction had been also changed drastically (log2 The The two in the inside the BR signal were considerably changed, nificantly (log2 two). 2). two genes genes BR signal pathway pathway have been drastically changed, se