We report the first instance of an acylthiourea-based sensor, N-(6-methoxypyridine-2-yl) carbamothioylbenzamide (NG1), forming panchromatic fluorescent fibres through self-assembly and exhibiting dual-sensing capabilities for the sequential detection of Cu²⁺ ions and lactic acid. The panchromatic fibres formed by NG1 undergo disaggregation upon exposure to Cu²⁺ ions, resulting in a visible color change from colorless to yellow. Subsequent addition of lactic acid induces re-aggregation into fibrillar structures, reversing the color back to colorless. This dynamic process enables NG1 to serve as a unique dual-sensor for sequential detection of both analytes. The sensing mechanism was thoroughly investigated using UV-visible and fluorescence spectroscopy. Two structural analogs, NG2 and NG3, were synthesized to elucidate the roles of the pyridine ring and acylthiourea side chain in panchromatic emission and metal binding. The O-methoxy group was found to significantly enhance sensitivity. The probe demonstrated excellent selectivity and sensitivity toward Cu²⁺ and lactic acid in vitro. Furthermore, its utility for cellular imaging was validated in human retinal pigment epithelial (RPE) cells, where it successfully enabled real-time visualization of intracellular Cu²⁺ and lactate levels.MYL4 Antibody Epigenetics Quantum chemical calculations based on density functional theory (DFT) provided theoretical support, confirming the binding interactions and explaining the observed photophysical changes.DDX5 Antibody Purity & Documentation To the best of our knowledge, this is the first report describing a single molecular probe capable of sequential detection of copper and lactate ions, with aggregation behavior directly linked to its sensing performance.PMID:34772595 This work opens new avenues for designing smart, responsive materials for biomedical diagnostics and environmental monitoring.
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