DsbA Enzyme

Description:
|Introduction DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli. |Reco|E. coli-derived. A periplasmic protein containing 208 amino acids having a molecular mass of 23,149 Dalton. Purity > 95%, by RP-HPLC and SDS-|Lyophilized after from a sterile solution containing 50 mM sodium phosphate buffer and 100 mM sodium chloride. Reconstitution: It is recommended to reconstitute the lyophilized DsbA in sterile 18M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Applications: WB. Shipping: The product is shipped at a|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.