With gDNA Eraser (TaKaRa, Tokyo, Japan). qPCR from the cDNA samples was performed employing a SYBR Green PCR kit (SYBR Premix Ex TaqTM II; TaKaRa). Paired primers have been made and are listed in Table S1. PCR was performed on a real-time PCR thermal cycler (qTOWER two.2; Analytik Jena, Jena, Germany) below the following circumstances: 95 for 2 min, followed by 40 cycles at 95 for 5 s, 55 for 30 s, and 72 for 30 s. The data were analyzed utilizing qPCRsoft 1.1 (Analytik Jena). The transcript quantification of every single gene was performed using at least three independent replicates. The relative fold-change in gene expression was normalized to that of elongation aspect NTR1 Modulator medchemexpress 1-alpha (ef-1) (BXY_0569100).Chen et al. BMC Genomics(2021) 22:Page 9 ofSupplementary informationThe on-line version includes supplementary material available at https://doi. org/10.1186/s12864-021-07714-y.eight.9. Added file 1. 10. Acknowledgements Thanks for the technical support from Dr. Linlin Pan (Beijing Biomedi Technologies Business) and Mr. Junhao Chen (Saint Louis University). Authors’ contributions Conceptualization, K.G. and X.Z.; methodology, S.C., F.L. and X.Z.; formal analysis, X.Z.; information curation, Y.C.; writing–original draft preparation, K.G.; writing–review and editing, X.Z. All authors have study and agreed to the published version in the manuscript. The author(s) read and authorized the final manuscript. Funding This study was funded by the National Organic Science Foundation of China(3187063731200487)and jointly funded by Zhejiang Essential Study Plan (2016C320162019C02024). S1PR5 Agonist site Availability of data and materials Availability of data and components, consist of details: Illumina sequence data have already been submitted to CNGBdb (https://db.cngb.org/) below the accession number CNP0001233. NCBI non-redundant protein (Nr) database: http://www.ncbi.nlm.nih.gov; Swiss-Prot protein database: http://www. expasy.ch/sprot; KEGG Database: https://www.kegg.jp/; KEGG PATHWAY. Database: https://www.kegg.jp/kegg/pathway; Gene Ontology Database: http://geneontology.org/; The DESeq2 and ClusterProfiler package are from R (https://bioconductor.org/). All data generated or analyzed through this study are incorporated within this published short article and its supplementary info files. approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare no conflict of interest. Received: 16 January 2021 Accepted: four May perhaps 2021 23. References 1. Zhao L, Jiang P, Humble LM, Sun J. Within-tree distribution and attractant sampling of propagative pinewood nematode, Bursaphelenchus xylophilus: An early diagnosis strategy. Forest Ecol Manag. 2009; 258: 1932937. two. Foit J, Cerm V, Gaar V, NovKH, RolincovP. New insights into the life history of Monochamus galloprovincialis can enhance surveillance tactics for the pinewood nematode. J Pest Sci. 2019; 92: 1203215. three. Futai K. Pine Wood Nematode, Bursaphelenchus xylophilus. Annu Rev Phytopathol. 2013; 51: 613. 4. Kikuchi T, Li H, Karim N, Kennedy M, Moens M, Jones J. Identification of putative expansin-like genes in the pine wood nematode, Bursaphelenchus xylophilus and evolution of your expansin gene family inside the Nematoda. Nematology. 2009; 11: 35564. five. Kang JS, Koh YH, Moon YS, Lee SH. Molecular properties of a venom allergen-like protein recommend a parasitic function inside the pinewood nematode Bursaphelenchus xylophilus. Int J Parasitol. 2012; 42: 630. six.